Journal: Journal of Virology

Article Title: Recovery of Infectious Virus by Transfection of In Vitro-Generated RNA from Tulane Calicivirus cDNA

doi: 10.1128/jvi.00696-08

Figure Lengend Snippet: FIG. 4. Plaque morphologies and growth kinetics of wild-type (WT) and in vitro-generated (TV) TV. (A) WT TV (a) and TV (b) exhibit similar plaque morphologies. (B) Growth curves of WT and TV viruses. LLC-MK2 cells were infected with WT or TV at a multiplicity of infection of 0.025.

Article Snippet: LLC-MK2 rhesus monkey kidney cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: In Vitro, Generated, Infection

Journal: Emerging Infectious Diseases

Article Title: Human Metapneumovirus, Peru

doi: 10.3201/eid1202.051133

Figure Lengend Snippet: Human metapneumovirus (HMPV)–positive samples by F- and N-gene primers

Article Snippet: A shell vial containing a near confluent monolayer of LLC-MK2 cells (Diagnostic Hybrids, Inc., Athens, OH, USA) was injected with 100 μL specimen and 900 μL HMPV growth media (1× minimum essential medium with L-glutamine and Earle salts, 0.1% bovine albumin, 1× HEPES, 0.001% porcine pancreatic trypsin, 0.4505 mol/L D-glucose, 10,000 U penicillin, 10 mg streptomycin, and 50 μg amphotericin), centrifuged for 1 h at 37°C and 2,800 rpm (1,500× g ), followed by a 37°C incubation with 5% CO 2 .

Techniques: